Fig. 2: ΔNp63α enriches the transcription of genes that promote EMT in EECs.
From: ΔNp63α-induced DUSP4/GSK3β/SNAI1 pathway in epithelial cells drives endometrial fibrosis
a Volcano plot representing DE genes in ΔNp63α (−) and ΔNp63α (+) EECs. Each plot represents a sample set. The statistical significance of all transcripts (p < 0.05 and fold change ≥ 2) was analyzed by Cuffdiff (v2.2.1). In the plot, green represents the downregulated transcripts and red represents the upregulated transcripts. b Heat map showing DE genes in ΔNp63α (−) and ΔNp63α (+) EECs. Each column represents a sample set. Each row represents an individual mRNA transcript. The statistical significance of all transcripts (p < 0.05 and fold change ≥ 2) was analyzed by Cuffdiff (v2.2.1). The heat map color spectrum corresponds to percentile ranks, indicating downregulated transcripts (green) and upregulated transcripts (red). c Major molecular functions of DE genes based on ontology analysis. GO analysis was performed using GO::Term Finder software. d Heat map of genes that promote EMT. Each column represents a sample set and each row represents an individual miRNA transcript. The heat map color spectrum corresponds to percentile ranks, showing downregulated transcripts (green) and upregulated transcripts (red). e qRT-PCR analysis of SNAI1, E-cadherin, N-cadherin, fibronectin (FN1), vimentin, α-SMA, GSK3β, and bFGF mRNA levels in EECs after incubation with Ad-ΔNp63α or Ad-CTL for 24 and 48 h (n = 3). *p < 0.05, and **p < 0.01.
