Fig. 1: Pancreatic β-like cell differentiation of hiPSCs using a five-stage protocol. | Cell Death & Disease

Fig. 1: Pancreatic β-like cell differentiation of hiPSCs using a five-stage protocol.

From: microRNA-181c-5p promotes the formation of insulin-producing cells from human induced pluripotent stem cells by targeting smad7 and TGIF2

Fig. 1

a Schematic strategy for the differentiation of hiPSCs into pancreatic β-like cells. DE, definitive endoderm; PG, primitive gut tube; PP, pancreatic progenitor; RA, retinoic acid. b Gene expression profile of hiPSC-derived cells at the S1-S5 stages of differentiation compared with hiPSCs. The data are expressed as the mean ± SD of three independent experiments (n = 6). *p ≤ 0.05, **p ≤ 0.001, relative to the hiPSCs control. c Representative flow cytometry plots illustrating the protein expression of CXCR4 and SOX17 (S1), PDX1 and NKX6.1 (S4), and PDX1 and insulin (S5) in populations of hiPSC-derived cells. d The dynamic expression levels of miR-375 and miR-181c-5p during the formation of IPCs. The data are expressed as the mean ± SD of three independent experiments (n = 8). **p ≤ 0.001, relative to the hiPSCs control. ns, not statistically significant. S1, stage 1; S2, stage 2; S3, stage 3; S4, stage 4; S5, stage 5.

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