Fig. 4: NORAD-4 rescues the apoptosis inhibition and tumor growth mediated by knockdown of NORAD in vitro and in vivo. | Cell Death & Disease

Fig. 4: NORAD-4 rescues the apoptosis inhibition and tumor growth mediated by knockdown of NORAD in vitro and in vivo.

From: NORAD orchestrates endometrial cancer progression by sequestering FUBP1 nuclear localization to promote cell apoptosis

Fig. 4

a, b The expression level of NORAD (a) and NORAD-4 (b) was detected by qRT-PCR in NORAD knockdown cell lines and in NORAD knockdown rescued by NORAD-4 fragment cell lines. c Cell-counting assays for the NORAD knockdown and rescued by NORAD-4 cell lines. d The expression levels of apoptotic associated markers were detected by western blot in the NORAD knockdown and rescued by NORAD-4 cell lines. e The percentage of apoptotic cells in the NORAD knockdown and rescued by NORAD-4 cell lines was analyzed by FACS. f SPEC-2-derived cell lines (sh-NORAD-1/2, sh-NORAD-1/2 + NORAD-4) were subcutaneously injected into the hind flanks of nude mice. g Tumor volume was monitored from day 0 to day 21 post injection. h Apoptosis in tumor tissues was presented by TUNEL assay. Scale bar, 10 μm. The results were determined from triplicates, and the error bars represented as the mean ± SD, */#P < 0.05, */##P < 0.01, ***/###P < 0.001, ****P < 0.0001.

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