Fig. 6: The NORAD/FUBP1 interaction results in the upregulation of downstream pro-apoptotic genes. | Cell Death & Disease

Fig. 6: The NORAD/FUBP1 interaction results in the upregulation of downstream pro-apoptotic genes.

From: NORAD orchestrates endometrial cancer progression by sequestering FUBP1 nuclear localization to promote cell apoptosis

Fig. 6

a RNA-seq data of liver cancer from TCGA to classify FUBP1 high- and low-expressed groups. b GSEA for the FUBP1-related pathways in liver cancer. c qRT-PCR analysis for the expression of four FUBP1 downstream targets (TRAIL, NOXA, BIK, and TNFA) by knockdown of FUBP1. d qRT-PCR analysis for the expression of four FUBP1 downstream target genes by the introduction of full-length NORAD or the NORAD-1 fragment, which was not bound to FUBP1. e, f ChIP-qPCR analysis for the FUBP1 (e) and RNA polymerase II (f) occupancies at the promoters of four target genes (TRAIL, NOXA, BIK, and TNFA) after transfecting full-length NORAD or NORAD-1 fragment. The fold enrichment was relative to the input DNA. The results were determined from triplicates, and the error bars represented as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001. TNF tumor necrosis factor, TRAIL TNF-related apoptosis-inducing ligand, NOXA PMAIP1, phorbol-12-myristate-13-acetate-induced protein 1, BIK BCL2 interacting killer, ChIP chromatin immunoprecipitation, Pol II RNA polymerase II.

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