Fig. 3: HIF-1α is associated with hypoxia-induced chondrocytes self-protection.
From: Stabilization of HIF-1α alleviates osteoarthritis via enhancing mitophagy
The chondrocytes were pretreated with HIF-1α target siRNA for 48 h followed hypoxia treatment (1% pO2) for 24 h. a The expression of HIF-1α was evaluated by western blots. b Quantification of HIF-1α immunoblots. c The expression of apoptotic proteins, including Bcl-2, Bax, Cyto C, and cleaved-caspase3 was evaluated by western blots. d Quantification of Bcl-2, Bax, Cyto C, and Cleaved-caspase 3 by immunoblots. e Mito-Sox was performed in chondrocytes. f The fluorescence intensity of Mito-sox in chondrocytes was quantified. g TUNEL assay was performed in chondrocytes. h The fluorescence intensity of TUNEL in chondrocytes was quantified. i JC-1 was measured in chondrocytes. j Representative image of immunofluorescence double staining of HIF-1α and Tom20 in chondrocytes. All data represent mean ± S.D. (n = 6). **P < 0.01, *P < 0.05.
