Fig. 5: DMOG promotes mitophagy and inhibits mitochondrial dysfunction and apoptosis through regulating HIF-1α expression in hypoxia stimulated chondrocytes.
From: Stabilization of HIF-1α alleviates osteoarthritis via enhancing mitophagy
The chondrocytes were pretreated with Si- HIF-1α for 48 h followed hypoxia treatment (1% pO2) and DMOG (2 mM) treatment for 24 h. a The expression of LC3-I, II, P62, BNIP3, Bcl-2, Bax, Cyto C and Cleaved-caspase3 from hypoxia treated chondrocytes were analyzed by western blot. b, c Quantification of LC3-I, II, P62, BNIP3, Cyto C, Bcl-2, Bax and Cleaved-caspase3 by immunoblots. d Representative image of immunofluorescence double staining of LC3 and Tom20 in chondrocytes. e Mito-Sox and TUNEL assay were performed in chondrocytes. f The fluorescence intensity of TUNEL in chondrocytes was quantified. g The fluorescence intensity of Mito-sox in chondrocytes was quantified. All data represent mean ± S.D. (n = 6). **P < 0.01, *P < 0.05.
