Fig. 1: Tfap2c is a facilitator of somatic cell reprogramming.

a AP-staining wells of a representative reprogramming experiment transduced with OSKM or OSKMT (day 9). b Flow-cytometer analysis of the SSEA1⁺ cells in reprogramming cells transduced with OSKM or OSKMT (day 9). c Flow-cytometer analysis of the Oct4-GFP⁺ cells in living cell population transduced with OSKM or OSKMT (day 12). d Flow-cytometer analysis of the Oct4-GFP⁺ cells in SSEA1⁺ cell population transduced with OSKM or OSKMT (day 12). e Image of Oct4-GFP⁺ colonies generated from MEFs transduced with OSKM or OSKMT in bright and fluorescent views (day 9). Scale bar, 100 μm. f Number of Oct4-GFP⁺ colonies of MEFs reprogrammed with OSKM and OSKMT at different time points. g AP-staining wells of a representative reprogramming experiment transduced with OSKM and either negative control shRNA or two shRNAs targeting Tfap2c (shT1 and shT2) on day 9. h Flow-cytometer analysis of the SSEA1⁺ cells in reprogramming cells in different groups (day 12). i Flow-cytometer analysis of the Oct4-GFP⁺ cells in living cell population in different groups (day 12). j Flow-cytometer analysis of the Oct4-GFP⁺ cells in SSEA1⁺ cell population in different groups (day 12). k Bright and fluorescent views of Oct4-GFP⁺ colonies in different groups (day 12). Scale bar, 100 μm. l Number of Oct4-GFP⁺ colonies in different groups at different time points. Significance in panels a, b, c, and d was determined by Unpaired two-tailed Student’s t-test. Significance in panels f and l was determined by two-way AVOVA with Sidak’s multiple comparisons test. Significance in panels i and j was determined by one-way ANOVA with Dunnett’s test. Significance summary: *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. All data are presented as mean ± S.D., N = 3.