Fig. 3: IL-1β and TNFα have synergistic effects on ETS1 expression during H. pylori infection. | Cell Death & Disease

Fig. 3: IL-1β and TNFα have synergistic effects on ETS1 expression during H. pylori infection.

From: Expression of ETS1 in gastric epithelial cells positively regulate inflammatory response in Helicobacter pylori-associated gastritis

Fig. 3

a ETS1 mRNA expression in AGS cells stimulated with H. pylori 11637(MOI = 100) and/or IFNγ, IL-17A, IL-22, IL-6, IL-12, or IL-23 (100 ng/ml) (24 h) were analyzed by real-time PCR (n = 3). The first three (IFNγ, IL-17A, and IL-22) are the results of the same batch, and the last three (IL-6, IL-12, and IL-23) are the results of another same batch. b, d ETS1 mRNA expression in AGS cells stimulated with H. pylori 11637(MOI = 100) and/or IL-1β (b) or TNFα (d) (100 ng/ml) (24 h) were analyzed by real-time PCR (n = 3). c, e ETS1 protein expression in AGS cells stimulated with H. pylori 11637(MOI = 100) and/or IL-1β (c) or TNFα (e) (100 ng/ml) (24 h) were analyzed by western blot. f, g AGS cells were pretreated with BAY 11-7082 (10 μM) and then treated with IL-1β for 24 h. ETS1 mRNA (f) and protein (g) expression were analyzed by real-time PCR (n = 3) and western blot. h, i AGS cells were pretreated with BAY 11-7082 (10 μM) and then treated with TNFα for 24 h. ETS1 mRNA (h) and protein (i) expression were analyzed by real-time PCR (n = 3) and western blot. Quantized expression of proteins were analyzed by Image Lab software (n = 1). n.s. P > 0.05, **P < 0.01.

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