Fig. 2: Oxidized ATM promotes TNBC-CSC enrichment.
Hs578T and BT549 cells, and ATM-silenced Hs578T and BT549 cells by transfectting two different shRNAs against ATM (shATM#1 and shATM#2) were suspensded in culture in CSC medium under normaxia (21% O2) or hypoxia (1% O2) and treated with or without ATM inhibitor KU60019 (ATM inhibitor, 10 µM). a The representative images of mammospheres are shown (scale bar, 100 µm). b The quantification of mammosphere formation efficiency. Data are shown as the mammospheres number/1000 seeded cells ± SEM from three independent experiments (**P < 0.01) (Nor normoxia, Hyp hypoxia). c The quantification of mammosphere sizes. The data represent means ± SD from three independent experiments (*P < 0.01, hypoxia vs normoxia; **P < 0.01, vs hypoxic control). d The representative images of mammospheres formed from breast tumor with low oxidized ATM and high oxidized ATM (scale bar, 100 µm) under normoxia or hypoxia condition and treatment with or without KU60019 (10 μM). e The mammosphere sphere number and mean sphere sizes of d were quantified (*P < 0.05, **P < 0.01).
