Fig. 1: ECRG2 expression is induced by DNA damage.
From: ECRG2, a novel transcriptional target of p53, modulates cancer cell sensitivity to DNA damage
a ECRG2 mRNA levels are induced by etoposide (Etop). ECRG2 mRNA was analyzed by quantitative real-time PCR (qRT-PCR). b ECRG2 protein levels are induced by etoposide (Etop). Western blot (WB) analyses were performed using the antibodies specific for ECRG2 (upper), p53 (middle), and vinculin (lower). Numbers indicate fold induction in ECRG2 protein levels and were obtained by normalizing the relative band intensities of ECRG2 to that of vinculin (loading control). Ponceau S staining images corroborate total protein loading indicated by vinculin. c–e ECRG2 regulation by various stress including agents that induce or do not induce DNA damage. Melph melphalan. TG thapsigargin. SD sulindac sulfide. UVC ultraviolet C. qRT-PCR data in (a, c, d, e) are presented as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.
