Fig. 5: Echinatin inhibits migration and invasion in ESCC cells.

The migration (a) and invasion (b) abilities of KYSE30 and KYSE270 cells treated with echinatin at different concentrations for 24 h were determined by chamber migration and invasion assays. c Western blot analysis of expressions of vimentin, β-catenin and E-cadherin in the KYSE30 and KYSE270 cells treated with echinatin at the indicated concentrations for 24 h. d, e The KYSE30 and KYSE270 cells transfected with AKT (T308D/S473D)-expressing plasmid or vector control were treated with echinatin at the indicated concentrations for 24 h, and then compared for migration ability (d), invasion ability (e). Bars, SD; *P < 0.05, **P < 0. 01, ***P < 0.001.