Fig. 3: CSF1R inhibition reduces microglial accumulation induced by SCI. | Cell Death & Disease

Fig. 3: CSF1R inhibition reduces microglial accumulation induced by SCI.

From: Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice

Fig. 3

a Schematics of experimental design showing the timeline of microglia depletion, spinal cord crush injury, and sacrifice. b Western blot analysis of spinal cord homogenates from sham-operated, injured with control or PLX3397-treated mice (n = 4 per group) for steady state levels of the microglia marker Iba1. c Quantification of b showing a robust increase in levels of Iba1 expression at 7 days following SCI. CSF1R inhibition reverses the increase induced by SCI. d–f Representative immunofluorescence images of mouse spinal cord sections showing Iba1+ microglia. The changes in number of Iba1+ microglia consist with the changes of Iba1 expression levels. Data are expressed as mean ± SD. Scale bars: e, f in f 100 µm.

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