Fig. 7: UBE2T regulated liver CSCs through the Wnt/β-catenin signaling cascade. | Cell Death & Disease

Fig. 7: UBE2T regulated liver CSCs through the Wnt/β-catenin signaling cascade.

From: The interplay of UBE2T and Mule in regulating Wnt/β-catenin activation to promote hepatocellular carcinoma progression

Fig. 7

a A tissue microarray consisting of 51 tumor tissues and corresponding nontumor liver tissues was subjected to IHC analysis. Cases #23 and #45 showed high expression of both UBE2T and β-catenin, while cases #15 and #20 showed low expression of these proteins. b UBE2T expression was significantly correlated with β-catenin expression in a cohort of 51 HCC clinical samples (***p < 0.001, Fisher’s exact test). c The addition of CHIR99021 at a dose of 1 μM abolished the inhibitory effect of UBE2T knockdown on the size and number of spheres formed from PLC/PRF/5 cells (*p < 0.05, t test). d The addition of CHIR99021 at a dose of 1 μM increased the expression of CD47, CD90, and CD133 upon UBE2T knockdown in PLC/PRF/5 cells (*p < 0.05 and **p < 0.01, t test). FACS and sphere formation assay represent means ± SD in at least three independent experiments (n = 3–4).

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