Fig. 1: Conditional knockout of VMP1 in the mDAergic neurons. | Cell Death & Disease

Fig. 1: Conditional knockout of VMP1 in the mDAergic neurons.

From: Essential role for autophagy protein VMP1 in maintaining neuronal homeostasis and preventing axonal degeneration

Fig. 1

A A simplified scheme of the experimental timeline for the administration of TAM to both VMP1cWT and VMP1cKO mice. B IFC analysis for VMP1 expression in the SNc was performed by using an antibody against VMP1 (Green) together with TH (Red) in VMP1cWT and VMP1cKO mice. The nuclei were labeled with DAPI (Blue). Scale bar, 10 μm. C The percentage of TH+ and VMP1+ neurons in the SNc of VMP1cWT and VMP1cKO mice (N = 3 mice per genotype). D WB analysis for the VMP1 expression levels in the midbrain and striatum. E Quantification of WB analysis of (D) (N = 3 mice per genotype). C Was analyzed by using Student’s t-test. E Was analyzed by using two-way ANOVA followed by Sidak’s multiple comparisons test. Data were represented as mean ± SEM. ***p < 0.001, ****p < 0.0001.

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