Fig. 7: The morphological alterations of RER in the mDAergic neurons of VMP1cKO mice. | Cell Death & Disease

Fig. 7: The morphological alterations of RER in the mDAergic neurons of VMP1cKO mice.

From: Essential role for autophagy protein VMP1 in maintaining neuronal homeostasis and preventing axonal degeneration

Fig. 7

A The representative TEM images of the observed RER. Scale bar, 0.5 μm. B The mean width of RER tubules was shown (N = 487 RER collectively counted from 3 VMP1cWT mice, N = 500 RER collectively counted from 3 VMP1cKO mice). C The proportion of RER tubules (>100 nm) was quantified from (B). D Double-label immunofluorescence of SEC31A (green) and TH (red) in the mDAergic neurons. The nuclei were labeled with DAPI (blue). Scale bar, 10 μm. E Quantification of the number of SEC31A+ puncta (>0.1 μm2) per TH+ neuron (N = 3 mice per genotype). Data were analyzed by using Student’s t-test and were represented as mean ± SEM. **p < 0.01, ****p < 0.0001. RER rough endoplasmic reticulum, NM nuclear membrane, RER rough ER.

Back to article page