Fig. 1: Epithelial–mesenchymal transition (EMT) was induced in tongue squamous carcinoma cell lines by LPS from Porphyromonas gingivalis. | Cell Death & Disease

Fig. 1: Epithelial–mesenchymal transition (EMT) was induced in tongue squamous carcinoma cell lines by LPS from Porphyromonas gingivalis.

From: LncRNA LTSCCAT promotes tongue squamous cell carcinoma metastasis via targeting the miR-103a-2-5p/SMYD3/TWIST1 axis

Fig. 1

a The morphological changes in CAL27 and SCC15 cells after P.g-LPS induction from a tightly connected epithelial phenotype to an evacuated interstitial phenotype (×200). b Western blot analyses showed that E-cadherin protein expression was decreased in CAL27 and SCC15 cells induced by P.g-LPS, whereas vimentin and the EMT-related transcription factor Twist1 protein levels were increased. c Immunofluorescence also showed a decrease in the epithelial marker E-cadherin and an increase in the interstitial marker vimentin. d Transwell invasion and metastasis assays confirmed that P.g-LPS-induced CAL27 and SCC15 cells showed higher invasion and metastasis than the control cells. Error bars represent the mean±S.D from three independent experiments. (**P < 0.01).

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