Fig. 2: Expression and clinical value of LTSCCAT in tongue squamous cell carcinoma.

a Heat maps were generated based on the sequencing results, showing the expression levels of LncRNA in CAL27 and CAL27/LPS cells (unsupervised cluster analysis). Each row represents one LncRNA, the left column represents the expression of CAL27 cells, and the right column represents the expression of P.g-LPS-induced CAL27 cells. Red represents a high expression level, and blue represents a low expression level. b According to the sequencing results of LncRNAs, LTSCCAT located 113 bp downstream of the Twist1 gene has a homeostatic effect on Twist1 expression. c The sequencing results were verified in tongue squamous carcinoma cell lines and showed that P.g-LPS significantly upregulated the expression of LTSCCAT in CAL27, SCC15, SCC9, and SCC25 cells. Error bars represent the mean±S.D. from three independent experiments. (**P < 0.01). d Nuclear pulp separation experiments showed that LTSCCAT was predominantly expressed in the cytosol. e Representative images of in situ hybridization for LTSCCAT in TSCC with periodontitis tissues and non-periodontitis tissues (scale bar: 50 µm). f The expression of LTSCCAT in clinical specimens was detected by RT-QPCR, indicating that LTSCCAT expression is higher in patients with periodontitis than in those without periodontitis. (**P < 0.01). g Kaplan–Meier analysis revealed that high expression of LTSCCAT is associated with poor prognosis. The p values were assessed by the log-rank test. (**P < 0.01).