Fig. 5: Steady state and stress-induced platelet production is normal in the absence of Mlkl.

a Percentage of CD41⁺ cells (megakaryocytes) in WT and Mlkl^−/− BM assessed via flow cytometry. WT n = 6 and Mlkl^−/− n = 6. b Ploidy distribution profile of CD41/PI-positive BM cells in WT and Mlkl^−/− mice as determined by flow cytometry, WT n = 6 and Mlkl^−/− n = 6. c Platelet surface receptor expression by mean fluorescence intensity (MFI) of GPIX, GPIbα, GPVI and CD41 were assessed on purified platelets from WT and Mlkl^−/− mice by flow cytometry, WT n = 3 and Mlkl^−/− n = 3. d Platelet counts and e percentage of thiol orange (TO)-positive reticulated platelets in WT and Mlkl^−/− mice post anti-platelet serum (APS) administration. Cohorts of mice were bled at the indicated time points, 72 h control n = 4, WT + APS n = 5, Mlkl ^−/− + APS n = 4; 120 h control n = 4, WT + APS n = 4 and Mlkl ^−/− + APS n = 5. f Representative western blot analysis of protein lysates from Mlkl^fl/fl or Mlkl^Pf4Δ/Pf4Δ purified blood platelets probed for MLKL. GAPDH was used as a loading control. g Megakaryocyte numbers per FOV in H&E-stained sections of BM from Mlkl^Pf4Δ/Pf4Δ and Mlkl^fl/fl control mice, n = 5 per genotype. h Representative images of H&E stained sternal sections from Mlkl^Pf4Δ/Pf4Δ and Mlkl^fl/fl control mice. Megakaryocytes are indicated by asterisks. n = 5 mice per genotype. Data are presented as mean ± s.d, student’s unpaired t-test, no significant differences.