Fig. 3: Knockdown of Pontin inhibits GBM cell growth by inducing cell cycle arresting.
From: The ATPase Pontin is a key cell cycle regulator by amplifying E2F1 transcription response in glioma
A Western blot of Pontin expression in U87MG, U251 cells, and LN229 transfected with indicated siRNAs (si-nc, si-Pontin-1, or si-Pontin-2). B IF detection of the expression and subcellular location of Pontin in U87MG and U251 as transfected in (A). Scale bar, 10 μm. C The growth curves of the indicated cells assessed by MTS assays. D FCM cell cycle analysis results of the indicated cells (left) and the percentages of cells in each phase (right). All the experiments were performed at least in triplicate. Data in (A), (C), and (D) are presented as the mean ± SD. ***P < 0.001.
