Fig. 5: Linc00707 regulated tumor-induced VM via binding to miR-651-3p.

A The predicted miR-651-3p binding sites in the 3′UTR of linc00707 (linc00707-3′UTR-Wt) and the designed mutant sequence (linc00707-3′UTR-Mut) were indicated. Luciferase reporter assay of HEK-293T cells co-transfected with linc00707-3′UTR-Wt or linc00707-3′UTR-Mut and the indicated miRNA. Data are presented as the mean ± SD (n = 3, each group). **P < 0.01 vs. linc00707-Wt + pre-NC group. B miR-651-3p was identified in the linc00707-RISC complex. Relative expressions of linc00707 and miR-651-3p were measured using qRT-PCR. Data are presented as the mean ± SD (n = 3, each group). **P < 0.01 vs. anti-IgG group. C Expression levels of miR-651-3p regulated by linc00707 in U87 and U251 cells. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. linc00707(−)-NC group. D–F CCK-8 assay, transwell, and three-dimensional culture were applied to evaluate the proliferation, migration, invasion, and tube formation effect of miR-651-3p and linc00707. The scale bar represents 50 µm. G The expressions of VM formation-related proteins of U87 and U251 cells after transfected with linc00707 and miR-651-3p plasmid was shown. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05, **P < 0.01 vs. linc00707(−)-NC + pre-NC group.