Fig. 6: SP2 regulated the expression of VM formation-related proteins at the transcriptional level. | Cell Death & Disease

Fig. 6: SP2 regulated the expression of VM formation-related proteins at the transcriptional level.

From: HNRNPD interacts with ZHX2 regulating the vasculogenic mimicry formation of glioma cells via linc00707/miR-651-3p/SP2 axis

Fig. 6

A Expression levels of SP2 in glioma tissues. Data are presented as the mean ± SD (n = 9, each group). *P < 0.05 vs. NBTs group; #P < 0.05 vs. LGGTs group. B Expression levels of SP2 in glioma cells. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. HA group. C–E CCK-8 assay, transwell, and three-dimensional culture were applied to evaluate the proliferation, migration, invasion, and tube formation effect of SP2 on U87 and U251 cells. Representative images and corresponding statistical plots are shown. The scale bar represents 50 µm. F Protein levels of MMP2, MMP9, and VE-cadherin regulated by SP2 in U87 and U251 cells. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05, **P < 0.01 vs. SP2(+)-NC group; #P < 0.05, ##P < 0.01 vs. SP2(−)-NC group. G–I Schematic representation of MMP2, MMP9, and VE-cadherin promoter region in 3000 bp upstream of the transcription start site (TSS) designated as +1.Putative SP2 binding sites are shown.

Back to article page