Fig. 7: miR-651-3p regulated tumor-induced VM formation via binding to SP2. | Cell Death & Disease

Fig. 7: miR-651-3p regulated tumor-induced VM formation via binding to SP2.

From: HNRNPD interacts with ZHX2 regulating the vasculogenic mimicry formation of glioma cells via linc00707/miR-651-3p/SP2 axis

Fig. 7

A The predicted miR-651-3p binding sites in the 3′UTR of SP2 (SP2-3′UTR-Wt) and the designed mutant sequence (SP2-3′UTR-Mut) were indicated. Luciferase reporter assay of HEK-293T cells co-transfected with SP2-3′UTR-Wt or the SP2-3′UTR-Mut and the indicated miRNA. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. pre-NC + SP2-3′UTR-Wt group. B Expression levels of SP2 regulated by miR-651-3p in U87 and U251 cells. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05, **P < 0.01 vs. pre-NC group; #P < 0.05 vs. anti-NC group. C The expression of SP2 in U87 and U251 cells after co-transfection with linc00707 and miR-651-3p plasmid was shown. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. linc00707(−)-NC + pre-NC group. D–F CCK-8 assay, transwell, and three-dimensional culture were applied to evaluate the proliferation, migration, invasion, and tube formation effect of miR-651-3p and SP2. The scale bar represents 50 µm. G The expression of VM formation-related proteins in U87 and U251 cells after co-transfection with miR-651-3p and SP2 plasmid was shown. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05,**P < 0.01 vs. pre-NC + SP2(+)-NC group; #P < 0.05, ##P < 0.01 vs. pre-NC + SP2(+) group.

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