Fig. 4: Tan IIA inhibits Aurora B activity and induces G2/M phase cell cycle arrest in OSCC cells.
From: Targeting Aurora B kinase with Tanshinone IIA suppresses tumor growth and overcomes radioresistance
A IB analysis of p-Aurora B and p-Histone H3 Ser10 in Tan IIA-treated CAL27 and SCC25 cells with various doses for 24 h. B IB analysis of p-Aurora B and p-Histone H3 Ser10 in 2 μM Tan IIA-treated CAL27 and SCC25 cells for different time points. C and D Immunofluorescence (IF) analysis of the phosphorylation of Histone H3 Ser10 in Tan IIA-treated CAL27 (C) and SCC25 cells (D); n = 3 independent biological replications, one-way ANOVA. ***p < 0.001. Scale bar, 10 μm. E and F Cell cycle analysis by flow cytometry of Tan IIA-treated CAL27 (E) and SCC25 cells (F); n = 3 independent biological replications, Student’s t test. ***p < 0.001. G IF analysis of the population of polyploidy cells in CAL27 cells treated with Tan IIA for 48 h. Scale bar, 5 μm; n = 3 independent biological replications, Student’s t test, ***p < 0.001.
