Fig. 2: Alternative Splicing in sphingolipid genes generate a breast cancer subtype-specific signature.

A–D Number of AS events in sphingolipid-metabolizing genes in breast cancer subtypes (TCGA-BRCA dataset) compared to normal-like breast tissue samples, as shown by differential usage of cassette exons (CE) (A, B) or by intron retention (IR) (C, D) events. Total number of Cassette Exon (CE) (B) and Intron Retention (IR) (D) events are shown for Luminal A, Luminal B, HER2+, and Basal subtypes, and total number of genes that they correspond to (A, C). Statistical test Irwin–Hall p-value summarization was used on individual splicing events. E Venn diagram showing the number of common and unique AS events in sphingolipid genes in different breast cancer subtypes (TCGA-BRCA dataset). F, G List of all CE (F) and IR events (G) in different sphingolipid-metabolizing genes in different breast cancer subtypes suggesting a subtype-specific AS signature.