Fig. 4: Identification of endogenous proteins translated from full-length PC and alternatively spliced AS1, CERS2 transcripts in BT-474 cells. | Cell Death & Disease

Fig. 4: Identification of endogenous proteins translated from full-length PC and alternatively spliced AS1, CERS2 transcripts in BT-474 cells.

From: Alternative splicing of ceramide synthase 2 alters levels of specific ceramides and modulates cancer cell proliferation and migration in Luminal B breast cancer subtype

Fig. 4

A Table representing the unique peptides corresponding to junction region for full-length (PC/FL, Exon 7–8) and spliced form (AS1/SF, Exon 7–9) of CERS2 proteins (NP = not present). B Diagrammatic representation of MS identified peptides corresponding to PC/FL- and AS1/SF-derived proteins. Dark red circle denotes the proteins identified by Peptide Shaker database and blue circles denote all shared peptides identified from the chymotrypsin digested samples in both PC/FL and AS1/SF proteins. Unique peptides covering the junction region for PC/FL or AS1/SF proteins are denoted by light red and green respectively. C MRM spectra for PC/FL (Exon 7–8) transcript-derived peptide (SIASDVKRKDF, 196–206 amino acids) showing daughter ions generated by fragmentation of parent ion (633.346 Da m/z). Inset showing distribution and intensity of daughter ions generated. D, E Peak area representing abundance of few selected daughter ions generated from parent ion (633.346 Da m/z) corresponding to PC/FL. F MRM spectra for AS1/SF (Exon 7–9) transcript-derived peptide (SIASDVKRKSAKMF, 196–209 amino acids) showing daughter ions generated from the parent ion (784.434 Da m/z). Inset showing distribution and intensity of daughter ions generated. G, H Peak area representing abundance of few selected daughter ions as quantified from parent ion (784.434 Da m/z) corresponding to AS1/SF protein (PC/FL = protein coding/full-length; AS1/SF = alternatively spliced 1/spliced form).

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