Fig. 4: UNC1999 increased the expression of miRNAs that regulate methionine cycling-associated genes.
From: A distinct metabolic response characterizes sensitivity to EZH2 inhibition in multiple myeloma
a–e ChIP-qPCR analysis of H3K27me3 enrichment in UNC1999-treated INA-6 cells on exonic and gene body regions of a miR-494-3p, b miR-130a-3p, c miR-134-5p, d miR-192-5p and e miR-4429. For each miRNA, we analysed three genomic regions (GR-1, GR-2, GR-3). The location amplified by each primer pair is shown below every graph. Statistical analysis was performed with two-way ANOVA. GATA2 and GAPDH were used as positive and negative controls for H3K27me3 enrichment, respectively. The experiments were performed in three biological replicates. Values: mean with SEM. f RT-qPCR analysis of five PRC2-targeted miRNAs in INA-6 cells, post-UNC1999-treatment. Data were generated in three biological replicates. Value: mean with SEM. g–j MCF7 cells were transfected with 5 nM of miR-494-3p, miR-130a-3p, miR-134-5p, miR-4429 and miR-192-5p mimics. RT-qPCR analysis of methionine cycling-associated genes after 48 h of miRNA mimic treatment for g MAT2A, h MAT2B, i CBS and j CTH. miR-130a-5p or miR4429 were used as negative controls. Statistical analysis was performed with two-tailed t-test. Values: mean with SEM. Data were collected from three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
