Fig. 5: Sensitivity to EZH2 inhibition was associated with DNA damage, G2 arrest and apoptosis.
From: A distinct metabolic response characterizes sensitivity to EZH2 inhibition in multiple myeloma
a–b Flow cytometry analysis of apoptotic markers in (a) INA-6 and (b) U1996 cells upon UNC1999 treatment. c Representative western blot against γH2AX in INA-6 and U1996 cells and signal quantification of the western blot. Optical density was normalized against H4. The corresponding uncropped western blots can be found in Supplementary Fig. 9d. d–e Flow cytometry analysis of the cell cycle in (d) INA-6 and (e) U1996 cells upon UNC1999 treatment. Statistical analysis was performed with one-way ANOVA and multiple t-test. All experiments were performed in three biological replicates. Values: mean with SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
