Fig. 4: The effects of CHBP on MDSCs were dependent on EPOR.
From: CHBP induces stronger immunosuppressive CD127+ M-MDSC via erythropoietin receptor
A Detection of CD11b+Ly6G−Ly6Chigh M-MDSCs and CD11b+Ly6G+Ly6Clow G-MDSCs by flow cytometry. B Quantification analysis of the G-MDSCs and M-MDSCs proportions. C T-cell proliferation detected by CFSE. The ratios of G-MDSCs and T cells were 1:2, 1:4, 1:8, 1:16, and 1:32. D T-cell proliferation detected by CFSE. The ratios of M-MDSCs and T cells were 1:2, 1:4, 1:8, 1:16, and 1:32. E The expressions of iNOS and Arg-1 by qPCR. F The arginase-1 activity. G The protein expressions of iNOS and Arg-1 by western blot.
