Fig. 3: Atezolizumab induces the mitochondria-related apoptosis of OS cells by activating JNK pathway.
From: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma
A–D Expression of mitochondria-related apoptosis protein in HOS and 143B was evaluated by western blot after the treatment of atezolizumab (10 µg/ml) with or without SP600125 (10 μM); E Representative immunofluorescence staining images of cleaved-caspase-9 (green) and cleaved-caspase-3 (red) in HOS and 143B after the treatment of atezolizumab with or without SP600125; F–I Expression of phospho-JNK and JNK in HOS and 143B was detected by western blot after the treatment of atezolizumab with different concentrations. All data are from at least three independent experiments and are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
