Fig. 4: Atezolizumab induces autophagy in OS cells.
From: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma
A, B Expression of autophagy-related protein in HOS and 143B was evaluated by western blot after the treatment of atezolizumab with different concentrations; C, D Transmission electron microscopy of HOS and 143B after the treatment of atezolizumab (10 µg/ml) for 24 h; E, F Representative immunofluorescence staining images of LC3B in HOS and 143B after the treatment of atezolizumab (10 µg/ml) with or without mito-TEMPO (10 μM); G, H Expression of autophagy-related protein in HOS and 143B was evaluated by western blot after the treatment of atezolizumab with or without mito-TEMPO; I, J Representative images and analysis of GFP-RFP-LC3 transfected HOS and 143B after the treatment of atezolizumab with or without mito-TEMPO. All data are from at least three independent experiments and are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
