Fig. 5: Inhibition of autophagy by CQ enhances atezolizumab-induced apoptosis in OS cells.
From: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma
A Representative immunofluorescence staining images of cleaved-caspase-3 in HOS and 143B after the treatment of atezolizumab (10 µg/ml) with or without CQ (10 μM); B, C Expression of apoptosis-related protein in HOS and 143B was detected by western blot after the treatment of atezolizumab with or without CQ; D, E Apoptosis of HOS and 143B was detected by flow cytometry after the treatment of atezolizumab with or without CQ; F, G Representative images and quantification of TUNEL staining in HOS and 143B after the treatment of atezolizumab with or without CQ. All data are from at least three independent experiments and are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
