Fig. 6: Inhibition of autophagy by Atg-5 siRNA enhances atezolizumab-induced apoptosis in OS cells.
From: Targeting autophagy enhances atezolizumab-induced mitochondria-related apoptosis in osteosarcoma
A Expression of Atg-5 in HOS was detected by qRT-PCR after siRNA transfection; B, C Expression of Atg-5, Bax, Bcl-2, and cleaved-caspase-3 in HOS was detected by western blot after siRNA transfection; D, E Apoptosis of HOS was detected by flow cytometry after Atg-5 siRNA transfection; F, G Representative images and quantification of TUNEL staining in HOS after Atg-5 siRNA transfection. All data are from at least three independent experiments and are presented as the means ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.
