Fig. 3: Effect of Fer-1 treatment on cell viability and changes in ferroptosis-related molecules in TGF-β1-stimulated NRK-52E cells at 12 h.
From: Characterization of ferroptosis in kidney tubular cell death under diabetic conditions
A MTT assay revealed that administration of Fer-1 (100 µM) significantly abrogated the decrease in cell viability in cultured NRK-52E cells exposed to TGF-β1 (10 ng/ml). B The decrease in Slc7a11 and Gpx4 mRNA expression seen in TGF-β1-stimulated NRK-52E cells was significantly ameliorated after Fer-1 treatment. C The decrease in xCT and GPX4 protein expression seen in TGF-β1-stimulated NRK-52E cells was significantly attenuated after Fer-1 treatment. D The increase in total iron levels in TGF-β1-stimulated NRK-52E cells was significantly abrogated after Fer-1 treatment. E The increase in protein and mRNA expression levels of FTH1 in TGF-β1-stimulated NRK-52E cells was significantly attenuated after Fer-1 treatment. F Transmission electron microscope images of mitochondria. Red arrow indicates mitochondria cristae vanish. Blue arrowhead indicates outer mitochondria membrane rupture. As shown is representative of three independent replicates. One-way ANOVA and Bonferroni post hoc tests were used for statistical analysis. Error bars represent SD. Original magnification, ×150 for upper and ×600 for lower. Scale bar = 2000 µm (upper), 500 µm (lower). **P < 0.01; ***P < 0.001 versus Con group. #P < 0.05; ##P < 0.01; ###P < 0.001 versus TGF-β1 group.
