Fig. 4: Ferroptosis inhibitors attenuated glutathione concentration and lipid peroxidation in TGF-β1-stimulated NRK-52E cells after 12 h.
From: Characterization of ferroptosis in kidney tubular cell death under diabetic conditions
A The decrease in glutathione concentration seen in cultured NRK-52E cells exposed TGF-β1 (10 ng/ml) was significantly ameliorated by Fer-1 (10 and 100 µM) treatment. B Administration of Fer-1 significantly attenuated the increase in lipid peroxidation seen in TGF-β1-stimulated NRK-52E cells. C FACS evaluated BODIPY 581/591-C11 fluorescence intensity. D Lipid peroxidation assessed using Image-iT® revealed that the increase seen in cultured NRK-52E cells after exposure to TGF-β1 was significantly abrogated by Fer-1 treatment. As shown is representative of three independent replicates. One-way ANOVA and Bonferroni post hoc tests were used for statistical analysis. Error bars represent SD. Original magnification, ×40 for all. Scale bar = 20 µm. ***P < 0.001 versus Con group. #P < 0.05; ##P < 0.01; ###P < 0.001 versus TGF-β1 group.
