Fig. 1: Cisplatin or doxorubicin induces cleavage of GSDME in renal tubular epithelial cells in vitro and in vivo.
From: Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity
A, B Western blot analysis of GSDME in HK-2 cells treated with various concentrations of cisplatin (0, 5, 10, and 20 μM) for 48 h, and with 20 μM cisplatin for different times (0, 3, 6, 12, 24, and 48 h). C, D Western blot analysis of GSDME in HK-2 cells incubated with various concentrations of doxorubicin (0, 1, 2, and 4 μg/ml) for 48 h and 4 μg/ml doxorubicin for different times (0, 3, 6, 12, 24, and 48 h). E, F Serum creatinine and BUN detection of normal and cisplatin-treated mice after 72 h. G Hematoxylin–Eosin (HE) staining of control and cisplatin-treated mice. Scale bar, 50 μm. H–J Western blot analysis of Gsdme in mice and caspase 3 in control and cisplatin-treated mice. All data are presented as means ± SD from at least three independent experiments (n = 3 for in vitro experiment; n = 6 for in vivo experiment). ***p < 0.001 versus control group, ****p < 0.0001 using two-tailed Student’s t tests.
