Fig. 2: Z-DEVD-FMK decreases cisplatin- or doxorubicin-induced pyroptosis in HK-2 cells.
From: Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity
A, E Representative light microscopy images of HK-2 cells treated with cisplatin (20 μM) or doxorubicin (doxorubicin, 4 μg/ml) before or after Z-DEVD-FMK (100 μM) intervention. The red arrow shows bubbles emerging from the plasma membrane. Scale bar, 50 μm. Cytotoxicity and cell viability were detected using the LDH assay (B, F) and CCK-8 detection (C, G) in HK-2 cells induced by cisplatin (20 μM) or doxorubicin (4 μg/ml) in the presence or absence of Z-DEVD-FMK (100 μM). Western blot analysis of GSDME and caspase 3 (CASP 3) cleavage in cisplatin-treated (20 μM) (D) and doxorubicin-treated (4 μg/ml) (H) HK-2 cells in the presence or absence of Z-DEVD-FMK (100 μM). All data are presented as mean ± SD from three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 using one-way ANOVA followed by the Tukey’s method.
