Fig. 4: GSDME knockout alleviates cisplatin- and doxorubicin-induced pyroptosis in HK-2 cells.
From: Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity
A Western blot analysis of GSDME expression in HK-2 cells treated with px459-GSDME-KO plasmid. B, C Percentage of PI+ HK-2 cells were detected by flow cytometry in the normal group (NC) and GSDME knockout group (GSDME-KO) treated with cisplatin (20 μM) or doxorubicin (4 μg/ml). D, G Representative light microscopy images of HK-2 cells treated with cisplatin (20 μM) or doxorubicin (4 μg/ml) in NC and GSDME-KO group. The red arrow indicates bubbles emerging from the plasma membrane. Scale bar, 50 μm. Cytotoxicity and cell viability were determined using the LDH assay (E, H) and CCK-8 detection (F, I) in NC and GSDME-KO HK-2 cells treated with cisplatin (20 μM) or doxorubicin (4 μg/ml). J, K Western blot analysis of cleavage of GSDME and caspase 3 in cisplatin- (20 μM) or doxorubicin- (4 μg/ml) treated HK-2 cells in NC and GSDME-KO group. All data are presented as mean ± SD from three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 using one-way ANOVA followed by the Tukey’s method.
