Fig. 6: GSDME cleavage is induced by ERK and JNK signaling in HK-2 cells.
From: Caspase 3/GSDME-dependent pyroptosis contributes to chemotherapy drug-induced nephrotoxicity
A, F Western blot analysis of ERK and JNK phosphorylation in HK-2 cells incubated with cisplatin (20 μM) or doxorubicin (4 μg/ml) for 3 h. B, G Western blot analysis of the cleavage of GSDME and caspase 3 in cisplatin- (20 μM) or doxorubicin- (4 μg/ml) treated HK-2 cells for 48 h with or without pretreatment of U0126 (10 μM) and SP600125 (10 μM). Cytotoxicity and cell viability were determined using the LDH assay (C, H) and CCK-8 detection (D, I) in HK-2 cells treated with cisplatin (20 μM) or doxorubicin (4 μg/ml) for 48 h with or without pre-treatment of U0126 (10 μM) and SP600125 (10 μM). E, J Representative light microscopy images of HK-2 cells treated with cisplatin (20 μM) or doxorubicin (4 μg/ml) for 48 h with or without pre-treatment of U0126 (10 μM) and SP600125 (10 μM). The red arrow indicates bubbles emerging from the plasma membrane. Scale bar, 50 μm. All data are presented as mean ± SD from three independent experiments (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 using one-way ANOVA followed by the Tukey’s method.
