Fig. 1: p53 induces ARTS expression in cancer cells.
A, B Ectopic p53 induces ARTS mRNA (A) and protein (B) expression in H460 cells. Cells were transfected with the vector or increased doses of p53 plasmid followed by RT-qPCR or IB analysis. C, D Ectopic p53 induces ARTS mRNA (C) and protein (D) expression in HCT116p53+/+ cells. Cells were transfected with the vector or increased doses of p53 plasmid followed by RT-qPCR or IB analysis. E, F The p53-inducing agents elevate ARTS mRNA (E) and protein (F) levels in H460 cells. Cells were treated with 5-fluorouracil (10 μM), Doxorubicin (1 μM), or Inauhzin (2 μM) for 18 h followed by RT-qPCR or IB analysis. G, H The p53-inducing agents elevate ARTS expression in melanoma cell lines. Cells were treated with 5-fluorouracil (10 μM) or Doxorubicin (1 μM) for 18 h followed by IB analysis. I H2O2-induced oxidative stress increases ARTS expression. Cells were treated with H2O2 for the indicated time followed by IB analysis. J Wild-type p53, but not mutant p53-R175H, induces ARTS expression. Cells were transfected with plasmids as indicated followed by IB analysis. K Ablation of p53 reduces ARTS mRNA expression upon multiple stress conditions. Cells were transfected with p53 siRNA and treated with Inauhzin (2 μM), Cisplatin (10 μM), 5-fluorouracil (10 μM), or Nutlin-3 (20 μM) for 18 h before harvest for RT-qPCR analysis. L Ablation of p53 reduces ARTS protein expression upon DNA damage stress. Cells were transfected with p53 siRNA and treated with Doxorubicin 18 h before harvest for IB analysis.
