Fig. 6: Inhibition of pancreatic tumor growth in vivo by BSO and DPI combinatory treatment.
A–D Athymic nude mice (n = 32) were injected subcutaneously with 5 × 105 KPC cells on the right flank at the age of 9 weeks and randomly divided into four groups (n = 8 per group). Treatment started 9 days after KPC cell injection with PBS, 500 mg/kg BSO, 6 mg/kg DPI, or 500 mg/kg BSO plus 6 mg/kg DPI. When the tumor diameter reached 18 mm in a few mice, all mice were sacrificed as mandated by the animal care protocol, and tumor nodules were excised for subsequent statistic comparison in size and weight. A Body weight changes in all 32 mice starting from KPC cell injection. No difference was noted in all four treatment groups. Two-way ANOVA was used for statistical analysis. B Photographic gross comparison of athymic nude mice bearing KPC cell xenografts on the right flank. C Tumor volumes were measured throughout the experiment to evaluate the effect of drug treatment using the following equation: tumor volume (mm3) = L × W × (L + W)/2 × 0.526. Data presented are mean tumor volume in mm3 ± SD. Two-way ANOVA was used for statistical analysis. ****p < 0.0001. D Statistic analysis of tumor weight among four treatment groups. Data presented are mean tumor weight ± SD (gram). Student’s t test. **p < 0.01.
