Fig. 1: Fgf9 CKO in Olig1-Cre mice causes epilepsy. | Cell Death & Disease

Fig. 1: Fgf9 CKO in Olig1-Cre mice causes epilepsy.

From: Deletion of FGF9 in GABAergic neurons causes epilepsy

Fig. 1

A Schematic diagram describes the design of the floxed and CKO Fgf9 allele. Coding exons are shown in green boxes. B PCR analyses show the genotyping in the Fgf9loxP/loxPOlig1-cre (CKOOlig1), Olig1-Cre, and wild-type mice. C Western blot analyses demonstrate a decrease in FGF9 expression in the CKOOlig1 brain compared to that in Fgf9flox/flox (F/F) controls at 8 weeks of age. GAPDH was used as the internal loading control. D CKOOlig1 mice exhibited growth retardation at 8 weeks of age. Data are mean ± SEM for 10 mice per genotype. *P < 0.05; **P < 0.01, nonparametric Mann-Whitney test. E EEG recordings from CKOOlig1 mice showing spontaneous seizures. Data represent mean ± SEM of triplicate samples.

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