Fig. 4: CMS promotes HDFs differentiation in a Piezo1-mediated mechanism.

A, B Western blotting analysis of α-SMA in HDFs subjected (or not subjected) to mechanical stretch and treated (or not treated) with GsMTx4. C, D Western blotting analysis of α-SMA in HDFs subjected (or not subjected) to mechanical stretch and treated (or not treated) with Piezo1 knockdown. E, F Immunofluorescence analysis of α-SMA in HDFs subjected (or not subjected) to mechanical stretch and treated (or not treated) with GsMTx4. (Scale bar = 50 μm). G, H Immunofluorescence analysis of α-SMA in HDFs subjected (or not subjected) to mechanical stretch and treated (or not treated) with Piezo1 knockdown. (Scale bar = 50 μm). I, J Representative images and quantitative analysis of the collagen gel contraction assay in the context of GsMTx4 application. K, L Representative images and quantitative analysis of the collagen gel contraction assay in the context of Piezo1 knockdown. The “Contraction %” means the ratio of the area of each collagen gel to the area of the original well surface. M, N Western blotting analysis of fibronectin, collagen I, and collagen III in HDFs subjected (or not subjected) to mechanical stretch and treated (or not treated) with GsMTx4. O, P Western blotting analysis of fibronectin, collagen I, and collagen III in HDFs subjected (or not subjected) to mechanical stretch and treated (or not treated) with Piezo1 knockdown. For collagen I and fibronectin, two bands are included in densitometric quantitation. For collagen III, one band is included in densitometric quantitation. The results are expressed as the means with SD (n = 3). One-way ANOVA is used for all analysis. *P < 0.05, **P < 0.01, ***P < 0.005.