Fig. 2: Verification of Ring1b-interacting proteins. | Cell Death & Disease

Fig. 2: Verification of Ring1b-interacting proteins.

From: Distinct Ring1b complexes defined by DEAD-box helicases and EMT transcription factors synergistically enhance E-cadherin silencing in breast cancer

Fig. 2

A, B Ring1b interacts with DDX3X and DDX5, or with Snail1 and Twist2. The 293 T cells were transfected with plasmids as indicated for 24 h. Proteins were captured by IP and analyzed by western blot. C Heatmap of interaction between Ring1b-associated proteins. D Schematic depiction of Ring1b complexes. E–G Ring1b complexes verified in breast cell lines. Lysates from 10 A, 10A-Ring1b, MCF-7, and 231 cells were analyzed by IP followed by western blot. H Expression of endogenous Ring1b-associated proteins in breast cell lines. Total protein extractions from cells were analyzed by western blot.

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