Fig. 3: Ring1b cooperates with DDXs or EMT TFs to strengthen the inhibition of E-cadherin. | Cell Death & Disease

Fig. 3: Ring1b cooperates with DDXs or EMT TFs to strengthen the inhibition of E-cadherin.

From: Distinct Ring1b complexes defined by DEAD-box helicases and EMT transcription factors synergistically enhance E-cadherin silencing in breast cancer

Fig. 3

Proteins as indicated were stably overexpressed or knocked down in cells using lentivirus. A Clustering analysis of EMT-related genes’ expression influenced by Ring1b. Total RNAs isolated from 10A-control and 10A-Ring1b cells were analyzed by qRT-PCR. B Gene Ontology (GO) term analysis of EMT-related genes influenced by Ring1b. C Effect of Ring1b on epithelial and epithelial genes expression. Total RNAs isolated from 10 A and 231 cells were analyzed by qRT-PCR. D–K Effect of Ring1b complexes on E-cadherin expression in 10 A and 231 cells. Total protein and RNAs isolated from cells were analyzed for E-cadherin expression by western blot and qRT-PCR. Error bars represent the means ± SEM. Unpaired t-test is performed to indicate a statistically significant difference. ns, P ≥ 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.

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