Fig. 6: Ring1b-dependent epigenetic remodeling is associated with multiple epigenetic markers. | Cell Death & Disease

Fig. 6: Ring1b-dependent epigenetic remodeling is associated with multiple epigenetic markers.

From: Distinct Ring1b complexes defined by DEAD-box helicases and EMT transcription factors synergistically enhance E-cadherin silencing in breast cancer

Fig. 6

Proteins as indicated were stably overexpressed or knocked down in 10 A cells using lentivirus. IgG was used as control antibody. Immunoprecipitated DNA fragments were captured by antibodies as indicated. The probability of proteins binding to E-cadherin promoter was analyzed by ChIP-qRT-PCR. A, B Effect of HDAC1 and Ezh2 on E-cadherin transcription. C–E Effect of Ring1b complexes on epigenetic markers distribution on the E-cadherin promoter. F–H Effect of three proteins on epigenetic markers distribution on the E-cadherin promoter. Error bars represent the means ± SEM. Unpaired t-test is performed to indicate a statistically significant difference. ns, P ≥ 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.

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