Fig. 8: Ring1b and associated proteins predict breast cancer metastasis.

Mean integrated optical density (IOD) was used to evaluate expression of proteins in tissues. E-cadherin expression was determined by immunoreactive scoring system (− negative, + weak, ++ to +++ moderate and strong, respectively). A Single factor analysis of E-cadherin expression in cancer tissues. B Double factor analysis of E-cadherin expression in cancer tissues. C Correlation analysis of Ring1b and associated proteins in cancer tissues. D Double factor analysis of survival curves in cancer tissues. The data from TCGA atlas were analyzed by Kaplan–Meier method. Paired t-test is performed to indicate a statistically significant difference.