Fig. 2: MSCs-induced profound differentiation in CML cell line. | Cell Death & Disease

Fig. 2: MSCs-induced profound differentiation in CML cell line.

From: Establishment of a novel mesenchymal stem cell-based regimen for chronic myeloid leukemia differentiation therapy

Fig. 2

A K562 cells were cultured alone or co-cultured with MSCs for different times. The relative mRNA levels of differentiation genes and transcription factors were assessed by RT-qPCR (n = 3). B Morphological changes accompanied by megakaryocytic differentiation were confirmed by Wright–Giemsa staining of cells with or without MSCs culture. Scale bars, 50 μm. Comparison of the cell size in K562 cells prior to or after stimulation by MSCs. C The amounts of cell surface protein CD41 and CD42b were measured by flow cytometric. The amounts of K562 cell surface proteins were expressed as the geometric mean fluorescence intensity. All statistical data in this figure represented the mean ± SEM (*P <0.05, **P <0.01, ***P <0.001, ns, not significant, one-way ANOVA for multiple comparisons). M MSCs.

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