Fig. 5: AAV.ULK1.DN mediates long-lasting axonal protection after spinal cord injury. | Cell Death & Disease

Fig. 5: AAV.ULK1.DN mediates long-lasting axonal protection after spinal cord injury.

From: AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo

Fig. 5

A Scheme of experimental setup. AAV vectors were injected into both sensorimotor cortices 3 weeks before SCI. Five weeks after SCI, parasagittal spinal cord sections were obtained. AAV: transduction with adeno-associated viral vectors; SMC: sensorimotor cortex; CST: corticospinal tract; SCI: spinal cord injury; L: left; R: right; IMG: imaging; WM: white matter; GM: gray matter. B Representative images of parasagittal spinal cord sections, including the lesion site and rostral region, transduced with given AAV showing mCherry-positive axons (red) and staining for GFAP (green) and DAPI (blue) up to 3000 µm rostral to lesion. Asterisks indicate the lesion areas. Scale bar: 1000 μm. C Higher magnification images of areas at around 2000 µm distance from the lesion border. Arrows indicate intact axons. Scale bar: 100 µm. D Quantification of the axon number index (a ratio of intact axon numbers to the total number of transduced axons) at the indicated distances from the lesion border after transduction with AAV.CTRL (n = 6 animals) and AAV.ULK1.DN (n = 4 animals). Data are presented as single data points and means ± SEM. *P < 0.05, #P = 0.08, §P = 0.07, according to two-tailed unpaired t-test for each distance.

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