Fig. 7: AAV.ULK1.DN alters ERK, ROCK2, and RhoA signaling. | Cell Death & Disease

Fig. 7: AAV.ULK1.DN alters ERK, ROCK2, and RhoA signaling.

From: AAV-mediated inhibition of ULK1 promotes axonal regeneration in the central nervous system in vitro and in vivo

Fig. 7

Lysates were obtained from E18 rat cortical neurons on DIV 8 after transduction with AAV.ULK1.DN or AAV.mCherry. A–C Representative immunoblots of ERK1/2 (A), p-ERK1/2 (A), ROCK2 (B), RhoA (C) and the corresponding bands of the loading control GAPDH are shown. Quantifications of the band intensities of ERK1/2 (A-n = 6 independent cultures), p-ERK1/2 (A-n = 5 independent cultures), ROCK2 (B-n = 5 independent cultures), and RhoA (C-n = 4 independent cultures) normalized to GAPDH as loading control. CTR: control. RAP: addition of rapamycin (750 nM) 24 h before lysis. Data are presented as single data points and means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ns: no significant difference, according to one-way ANOVA and Tukey’s multiple comparisons test (A, B) or one-sample t-test (C).

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