Fig. 2: Cleaved Tau is observed in KI/Cre brains. | Cell Death & Disease

Fig. 2: Cleaved Tau is observed in KI/Cre brains.

From: Caspase-6-cleaved Tau fails to induce Tau hyperphosphorylation and aggregation, neurodegeneration, glial inflammation, and cognitive deficits

Fig. 2

a Western blot analyses of 20 μg total proteins from 2- and 9-month-old CTO KI/Cre, CTC KI/Cre, and WT/WT hippocampal (Hc) and cortical (Cx) proteins with anti-TauΔD13. b Western blot analyses with anti-TauΔD13, anti-hTau (N-term Tau), and anti-total Tau antibodies of heat-resistant proteins (HR) from human cerebellum lysate incubated with recombinant human active Casp6p20p10 (rCasp6) (+) or vehicle (−) for 1 h or 4 h. c TauΔD402 fragments were immunoprecipitated from 19-month-old Tau KO, WT/WT, CTO, and CTC KI/Cre brain lysates with the anti-TauΔD402 antibody, and analyzed by western blotting with anti-total Tau antibody. Recombinant hTau digested with rCasp6 (TauΔCasp6) was used as positive control. d Western blot analyses with anti-total Tau and anti-TauΔD421 antibodies of HR from human cerebellum or Casp6 KO mouse brainstem lysate incubated with rCasp6 (+) or vehicle (−) for 1 h or 4 h. e, f Representative micrographs of e CTC KI/Cre aged from 3 to 25 months (3-month-old: n = 5, 9-month-old: n = 5, 18-month-old: n = 4, 25-month-old: n = 3), and 25-month-old CTO KI/Cre (n = 4) and WT/WT (n = 3), or f 18-month-old CTC KI/Cre (n = 4) hippocampus (Hc), corpus callosum (CC), and cortex (Cx) tissue sections immunostained with e anti-TauΔD421 or f anti-∆Casp3 antibody. Sections from AOM-DSS-treated mouse colon and human fetal stomach were used as controls.

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